elisa kit  (ALPCO)

Journal: Molecules

Article Title: New Glycotoxin Inhibitor from Sesuvium sesuvioides Mitigates Symptoms of Insulin Resistance and Diabetes by Suppressing AGE-RAGE Axis in Skeletal Muscle

doi: 10.3390/molecules29153649

Figure Lengend Snippet: Effect of compound 1 on symptoms of diabetes in a mouse model of diabetes. ( A ) Glucose tolerance. ( B ) Insulin levels. ( C ) Insulin levels. ( D ) Adiponectin levels. ( E ) Insulin resistance. ( F ) Glycogen synthesis in muscles. Periodic acid–Schiff staining of gastrocnemius muscle. Results represent mean ± SEMs. n = 10–20 in each group; * p < 0.05, diabetic mice vs. treated with 1 .

Article Snippet: Cellular and serum AGEs, insulin, and adiponectin levels were measured by the quantitative sandwich enzyme immunoassay technique by using an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (STA-817, CML kit, San Diego, CA, USA), Mice insulin ELISA kit, and Mice adiponectin ELISA kit (DRP-300, R&D System, Minneapolis, MN, USA).

Techniques: Muscles, Staining

Journal: Antioxidants

Article Title: Therapeutic Potential of Cranberry Proanthocyanidins in Addressing the Pathophysiology of Metabolic Syndrome: A Scrutiny of Select Mechanisms of Action

doi: 10.3390/antiox14030268

Figure Lengend Snippet: Effect of PAC on insulin resistance, fasting lipid profile, lipoprotein composition, and inflammatory circulating biomarkers. At weeks 0, 6, and 10, ( A ) fasting glycemia and ( B ) fasting insulinemia was measured, enabling the calculation of the ( C ) Homeostatic model assessment of insulin resistance (HOMA-IR). At weeks 0, 6, 10, and 12, ( D ) fasting triglyceridemia and ( E ) fasting total cholesterolemia were measured. Results are presented as means ± SEM for n = 6–12 mice/group. At week 12, the lipid profile was further investigated to determine levels of ( F ) HDL-cholesterol and ( G ) non-HDL-cholesterol. Results are presented as means ± SEM for n = 4 pooled plasma/group. At week 12, plasma from n = 8 mice/group was collected and pooled for lipoprotein determination. Isolated fractions were first obtained via fast-protein liquid chromatography (FLPC) and then were analyzed to reveal content in ( H ) total cholesterol and ( I ) esterified cholesterol, as described in Materials and Methods. Circulating ( J ) adiponectin was measured at week 10. Circulating ( K ) interleukin-1-alpha (IL1A), ( L ) Monocyte Chemoattractant Protein-1 (MCP1), and ( M ) Tumor Necrosis Factor-alpha (TNFA) were obtained at week 12. Results are presented as means ± SEM for n = 6–8 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Chow; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. HFHF mice.

Article Snippet: Plasma samples ( n = 8 mice/group) obtained at week 10 were used to measure high molecular weight (HMW) adiponectin concentrations with the Mouse HMW and Total Adiponectin ELISA kit (ALPCO, Salem, NH, USA).

Techniques: Clinical Proteomics, Isolation, Fast Protein Liquid Chromatography

Journal: Nutrients

Article Title: Glycomacropeptide as an Efficient Agent to Fight Pathophysiological Mechanisms of Metabolic Syndrome

doi: 10.3390/nu16060871

Figure Lengend Snippet: Glycomacropeptide administration prevented systemic insulin resistance and inflammation in high-fat, high-fructose diet-fed mice. At weeks 0, 6 and 10, blood samples were obtained after a 12 h overnight fast to assess insulin resistance and dyslipidemia development in mice ( n = 8/group). ( A ) Glycemia, ( B ) insulinemia and ( C ) homeostatic model assessment of insulin resistance (HOMA-IR), ( D ) triglyceridemia and ( E ) total cholesterolemia. At the end of the 12-week experiment, plasma was collected to characterize mouse inflammatory profiles ( n = 8/group). The following cytokines were found to be modulated by GMP treatment: ( F ) MCP-1, ( G ) MIG, ( H ) MIP-2, ( I ) TNF-α and ( J ) RANTES. ( K ) Adiponectin concentration was also determined as described in the Materials and Methods section. Data are expressed as the mean ± SEM. Versus chow: * p < 0.05, **** p < 0.0001; versus HFHF + Bipro: # p < 0.05, ### p < 0.001, #### p < 0.0001. MCP-1: monocyte chemoattractant protein; MIG: monokine induced by gamma interferon; MIP-2: macrophage inflammatory protein; TNF-α: tumour necrosis factor alpha; RANTES: regulated on activation, normal T cell expressed and secreted.

Article Snippet: At sacrifice, plasma was collected to measure high molecular weight (HMW) adiponectin concentrations with the Mouse HMW & Total Adiponectin Elisa kit (ALPCO, Salem, NH, USA).

Techniques: Clinical Proteomics, Concentration Assay, Activation Assay